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Team:Hawaii/Notebook/2008-07-17
From 2008.igem.org
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=== Plasmid Prep=== | === Plasmid Prep=== | ||
| - | :<strong> Grace <strong> | + | :<strong> Grace </strong> |
| - | :* Inoculated 7 ml LB + amp<sub>100<sub> with single colony of B0015, R0010, J04430 for redo of plasmid prep tomorrow | + | :* Inoculated 7 ml LB + amp<sub>100</sub> with single colony of B0015, R0010, J04430 for redo of plasmid prep tomorrow |
=== Restreak to Purify=== | === Restreak to Purify=== | ||
Revision as of 03:38, 18 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
PCR
- Grace
- Colony PCR for GFP fusion brick and new pRL1383a to verify inserts
- Sequencing (25 mu;l reactions)
- nir promoter
- slr2016
- pilA
- BBa_B0015
- BBa_B0024
- BBa_B0030
- BBa_C0012
- BBa_E0040
- BBa_J04430
- BBa_J33207
- BBa_R0010
- Ran all PCR products on a 4% gel to check
- Gel ran funny -- loading dye ran 2x as fast as the DNA itself
- Poor ladder resolution on gel
- No bands seen for GFP fusion, pRL1383a, R0010
- Incorrect bands for B0015 and J33207
- Will redo plasmid preps for R0010, B0015, J33207
- Will redo PCR reaction tomorrow for pRL1383a, GFP fusion, B0015, J33207, R0010
Plasmid Prep
- Grace
- Inoculated 7 ml LB + amp100 with single colony of B0015, R0010, J04430 for redo of plasmid prep tomorrow
Restreak to Purify
- Grace<strong>
- Restreaked GFP fusion and pRL1383a (MCS replaced) transformants to purify
pSB3K3 plasmid prep
- <strong> Margaret
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
