Alberta NINT/18 July 2008

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lab work (SD): 

            The double-plasmid cell cultures showed one-two colonies on the LB+amp+kan35 plates.
            To test transformation efficiency, we will plate 150ul of XL1-B with pSB4A5+pSB4K5 on LB+amp50, 
            LB+kan35, and LB+amp50+kan35 plates using different amounts of plasmid and cells.
                              pSB4A5 (ul)   pSB4K5 (ul)   XL1-B (ul)
                  A              1.0           1.0           100
                  B              4.0           4.0           100
                  C              1.0           1.0            50
                  D              2.0           2.0            50


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