Team:Hawaii/Protocols/ExoSAP
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(New page: ==Overview== ExoSAP removes ssDNA and dNTPs ==Protocol== 1. Add 2 μl ExoSAP to 5 μl of the PCR reaction. 2. Incubate in a 37C water bath for 15 min. 3. Incubate in a 80C water bath f...)
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(New page: ==Overview== ExoSAP removes ssDNA and dNTPs ==Protocol== 1. Add 2 μl ExoSAP to 5 μl of the PCR reaction. 2. Incubate in a 37C water bath for 15 min. 3. Incubate in a 80C water bath f...)
Newer edit →
Revision as of 23:54, 18 July 2008
Overview
ExoSAP removes ssDNA and dNTPs
Protocol
1. Add 2 μl ExoSAP to 5 μl of the PCR reaction. 2. Incubate in a 37C water bath for 15 min. 3. Incubate in a 80C water bath for 15 min. to inactivate ExoSAP.