Team:BCCS-Bristol/Calendar-Notebook/1 July 2008

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then freeze at -20oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.
then freeze at -20oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.
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'''Swimming Medium'''
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''In 100ml of water'''
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| align="center" style="background:#f0f0f0;"|'''weight (g)'''
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|-
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| Potassium Phosphate||0.1742
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|-
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| NaCl||0.392
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|-
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| Glucose||0.18016
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|-
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| EDTA||0.003722
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|-
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| Tween -20 aka polysorbate 20||0.2 MICRO litres
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|-
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|
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|}

Revision as of 14:19, 24 July 2008

Home The Team The Project Submitted Parts Modelling Wet Lab Calendar Miscellaneous


This is a page for any solutions

Media for Plates 0.3% 400ml 0.15% 150ml 0.5% 150ml
Bactotryptone4g1.5g1.5g
NaCl2g0.75g0.75g
BactoAgar1.2g0.225g0.75g

in addition did a streak out for master colonies of MG1655 in LB from table below overnight at 37oC

10ml LB '
Yeast Extract0.05
Tryptone0.1
Nacl0.1



SOC medium in preparation for Biobrick transformation this consisted of

Trypton 2g
Yeast Extract0.5g
NaCl0.05g

in 95ml of water, then autoclaved. after this add this:

MgCl2 0.5ml 2M solution
Glucose2ml 1M

then freeze at -20oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.


Swimming Medium

In 100ml of water weight (g)
Potassium Phosphate0.1742
NaCl0.392
Glucose0.18016
EDTA0.003722
Tween -20 aka polysorbate 200.2 MICRO litres
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