Team:Johns Hopkins/Notebook/GROUP 6: Vectors

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(GROUP 6: Vectors =)
(GROUP 6: Vectors)
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   0.1 ng 5 * 10e7 <2 * 10e2<br>
   0.1 ng 5 * 10e7 <2 * 10e2<br>
   Preparative digests ready for use are located – where?
   Preparative digests ready for use are located – where?
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  [http://www.jhu.edu/iGEM/GroupOther:Other/2008-7-24.Vectors%20(CAM,AMP,KAN3,KAN4)%20after%20Digest.Tejas.html Vectors (CAM,AMP,KAN3,KAN4) after Digest]

Revision as of 23:07, 24 July 2008

GROUP 6: Vectors

 Status report by ____
 Vector transformed into bacteria strain DB3.1 Y/N
 Permanent culture made in the Boeke lab for future reference Y/N
 Selectable marker for this vector
 Medium made and tested Y/N  (link)
 DNA preps made Y/N
 DNA preps tested by RE digest  - (link)
 DNA preps tested by transformation into DB3.1 and DH5alpha (or JM109) – put data as 
   Table on moodle.  
 Sample format/data follows:
Amount transformed cfu/micGm in DB3.1 cfu/micGm in JM109 0.1 ng 5 * 10e7 <2 * 10e2
Preparative digests ready for use are located – where?


 [http://www.jhu.edu/iGEM/GroupOther:Other/2008-7-24.Vectors%20(CAM,AMP,KAN3,KAN4)%20after%20Digest.Tejas.html Vectors (CAM,AMP,KAN3,KAN4) after Digest]