Team:University of Washington/Project

From 2008.igem.org

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=== Bacterial Conjugation ===
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The bacteria will then build the conjugation machinery, in response to the AHL produced from the yeast.  This is being worked on in two separate ways on two different genes.
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Two major genes in regulating IncP conjugation are KorA, a global regulation located on the RP4 plasmid, and TrbA, a gene that produces a transcriptional factor that controls the transcription of the TrbB operon, which contains the essential genes for building the conjugation pili and other parts of the conjugation tube.
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[[Image:RP4 conjugation.gif|center]]
[[Image:RP4 conjugation.gif|center]]
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Illustration of Tra1 Conjugation Machinery<br\>
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<blockquote>'''Illustration of Tra1 Conjugation Machinery'''<br\></blockquote>
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Schröder, G., S. Krause, E. L. Zechner, B. Traxler, H. J. Yeo, R. Lurz, G. Waksman, and E. Lanka. 2002. TraG-like proteins of DNA transfer systems and of the Helicobacter pylori type IV secretion system: inner membrane gate for exported substrates? J. Bacteriol. 184:2767-2779. [http://www.ncbi.nlm.nih.gov/pubmed/11976307].
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<blockquote><p>Schröder, G., S. Krause, E. L. Zechner, B. Traxler, H. J. Yeo, R. Lurz, G. Waksman, and E. Lanka. 2002. TraG-like proteins of DNA transfer systems and of the Helicobacter pylori type IV secretion system: inner membrane gate for exported substrates? J. Bacteriol. 184:2767-2779. [http://www.ncbi.nlm.nih.gov/pubmed/11976307].</p></blockquote>
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<!--- The Mission --->
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<!---== Your abstract ==--->
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<!---== Project Details==--->
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<!---=== The Experiments ===--->
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<!---== Results ==--->
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== UW Lab Links ==
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{|align="justify"
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[[Team:University_of_Washington/Protocols| Protocols]]<br\>
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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[[Team:University_of_Washington/BioBrick Parts Used| BioBrick Parts Used]]<br\>
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|[[Image:Example_logo.png|200px|right|frame]]
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[[UW Glycerol Stocks]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Team.png|right|frame|Your team picture]]
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|align="center"|[[Team:University_of_Washington | Team Example 2]]
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<!--- The Mission, Experiments --->
 
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:University_of_Washington/Notebook|Notebook]]
!align="center"|[[Team:University_of_Washington/Notebook|Notebook]]
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
 
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== '''Overall project''' ==
 
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Your abstract
 
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== Project Details==
 
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=== Bacterial Conjugation ===
 
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The bacteria will then build the conjugation machinery, in response to the AHL produced from the yeast.  This is being worked on in two separate ways on two different genes.
 
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Two major genes in regulating IncP conjugation are KorA, a global regulation located on the RP4 plasmid, and TrbA, a gene that produces a transcriptional factor that controls the transcription of the TrbB operon, which contains the essential genes for building the conjugation pili and other parts of the conjugation tube.
 
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=== The Experiments ===
 
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=== Part 3 ===
 
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== Results ==
 
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== UW Lab Links ==
 
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[[Team:University_of_Washington/Protocols| Protocols]]<br\>
 
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[[Team:University_of_Washington/BioBrick Parts Used| BioBrick Parts Used]]<br\>
 
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[[UW Glycerol Stocks]]
 

Revision as of 05:31, 25 July 2008

Bacterial Conjugation

The bacteria will then build the conjugation machinery, in response to the AHL produced from the yeast. This is being worked on in two separate ways on two different genes.

Two major genes in regulating IncP conjugation are KorA, a global regulation located on the RP4 plasmid, and TrbA, a gene that produces a transcriptional factor that controls the transcription of the TrbB operon, which contains the essential genes for building the conjugation pili and other parts of the conjugation tube.

RP4 conjugation.gif
Illustration of Tra1 Conjugation Machinery

Schröder, G., S. Krause, E. L. Zechner, B. Traxler, H. J. Yeo, R. Lurz, G. Waksman, and E. Lanka. 2002. TraG-like proteins of DNA transfer systems and of the Helicobacter pylori type IV secretion system: inner membrane gate for exported substrates? J. Bacteriol. 184:2767-2779. [http://www.ncbi.nlm.nih.gov/pubmed/11976307].


UW Lab Links

Protocols
BioBrick Parts Used
UW Glycerol Stocks


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