Team:Hawaii/Construction of pRL1383a

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== Methods ==
== Methods ==
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#Transformation
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#Verification with Colony PCR
== Results ==
== Results ==

Revision as of 02:54, 26 July 2008

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Contents

Construction of pRL1383a

  • The BioBrick parts of pRL1383a are to be ligated in a series of experiments.

Methods

  1. Round 1 of Restriction Digest and Ligation
Restriction Digest & Ligation: Round 1
Name Digest 1 Digest 2 Ligation to Product
mob XbaI & PstI n/a [http://partsregistry.org/Part:BBa_B0024 BBa_B0024] TT-mob
rep XbaI & PstI n/a [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Plac-rep
oriV XbaI & PstI n/a [http://partsregistry.org/Part:pSB1A3 pSB1A3] pSB1A3::oriV
aadA region XbaI & PstI n/a [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Plac-aadA
oriT XbaI & PstI n/a [http://partsregistry.org/Part:pSB1A3 pSB1A3] pSB1A3::oriT
pSB1A3 XbaI & PstI n/a see oriT and oriV see oriT and oriV
[http://partsregistry.org/Part:BBa_B0024 BBa_B0024] SpeI PstI see mob region above see mob region above
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] SpeI PstI see rep and aadA region above see rep and aadA region above
  1. In gel purification of ligation products
  1. Round 2 of Restriction Digest and Ligation
Restriction Digest & Ligation: Round 2
Name Digest 1 Digest 2 Ligation to Product
TT-mob EcoRI SpeI [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] TT-mob-TT
Plac-aadA EcoRI SpeI [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] Plac-aadA -TT
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015] EcoRI XbaI see mob and aadA above see mob and aadA above
  1. Transformation
  1. Verification with Colony PCR

Results


Discussion

  • What was learned and how to do future experiments differently.


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]

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