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Team:Hawaii/Notebook/2008-07-23
From 2008.igem.org
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:<strong> Margaret </strong> | :<strong> Margaret </strong> | ||
[[Image:plasmid_prep.pdf|right|thumb|300px|Plasmid Prep of pSB1A3, pSB1A2, pSB3K3, and a verification of pSMC121.]] | [[Image:plasmid_prep.pdf|right|thumb|300px|Plasmid Prep of pSB1A3, pSB1A2, pSB3K3, and a verification of pSMC121.]] | ||
| + | |||
| + | ===Biobrick Extraction=== | ||
| + | :<strong>Margaret</strong> | ||
| + | |||
| + | :*extracted: I14032, pSB1A7, I51020, J23012 and transformed into DB3.1 cells. Plated on amp100. **the problem is that I14032 is Kan resistant. I introduced 1mM IPTG to the 2 hour 37°C incubation, but we will not see the results of this because of my antibiotics mix-up | ||
| + | :*on 7/24 there were 2 colonies on the pSB1A7 plate and 4 colonies on the I51020 plate, these were restreaked | ||
| + | :*on 7/25 the re-streaked plates were placed in 4°C. | ||
===Plasmid Prep pRL1383a-M=== | ===Plasmid Prep pRL1383a-M=== | ||
Latest revision as of 03:32, 26 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Gel for Plasmid Prep from Yesterday
- Margaret
File:Plasmid prep.pdf
Plasmid Prep of pSB1A3, pSB1A2, pSB3K3, and a verification of pSMC121.
Biobrick Extraction
- Margaret
- extracted: I14032, pSB1A7, I51020, J23012 and transformed into DB3.1 cells. Plated on amp100. **the problem is that I14032 is Kan resistant. I introduced 1mM IPTG to the 2 hour 37°C incubation, but we will not see the results of this because of my antibiotics mix-up
- on 7/24 there were 2 colonies on the pSB1A7 plate and 4 colonies on the I51020 plate, these were restreaked
- on 7/25 the re-streaked plates were placed in 4°C.
Plasmid Prep pRL1383a-M
- Krystle
- 200 ml prep of the new pRL1383 plasmid with biobrick multiple cloning site
Checked for transformants from yesterday/restreaked
- Krystle
- GFP + tt = 5 colonies
- GFP fusion + tt = 15 colonies
- nir + rbs = 1 colony
Discussion
Quote of the Day
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
