Minnesota/27 July 2008

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|'''j.''' (10) BV:Tet/p22 #2 = [DNA] ng/uL is 15, [DNA] ug/uL is 0.015    LOW/POOR
|'''j.''' (10) BV:Tet/p22 #2 = [DNA] ng/uL is 15, [DNA] ug/uL is 0.015    LOW/POOR
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|-
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|'''2. Digest Rxn:''' Using #'s: 1, 2, 6, 9, and 10 from above. Also using: p22cII, LAMBDAcI, RFP from different plasmid prep product days. Follow the table below:
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|'''2. Digest Rxn:''' Using #'s: 1, 2, 6, 9, and 10 from above. Follow the table below:
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{|border="1" align="left"
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|-
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!| Parts !! 10x Buffer !! BSA !! DNA !! RE 1 !! RE 2 !! H20
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|-
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| BV:Lac I (1) || 5.0uL || 0.5uL || 22.0uL || Spe1, 1.0uL || Pst1, 1.0uL || 20.5uL
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|-
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| BV:Lac I #2 (2) || 5.0uL || 0.5uL || 28.5uL || Spe1, 1.0uL || Pst1, 1.0uL || 14.0uL
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|-
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| BV:Tet  (6) || 5.0uL || 0.5uL || 35.0uL || Spe1, 1.0uL || Pst1, 1.0uL || 7.5uL
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|-
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| BV:Tet/p22 #1 (9) || 5.0uL || 0.5uL || 3.0uL || Spe1, 1.0uL || Pst1, 1.0uL || 39.5uL
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|-
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| BV:Tet/p22 #2 (10) || 5.0uL || 0.5uL || 33.0uL || Spe1, 1.0uL || Pst1, 1.0uL || 9.5uL
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|-
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| p22cII || 5.0uL || 0.5uL || 5.0uL || Xba1, 1.0uL || Pst1, 1.0uL || 37.5uL
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|-
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| LAMBDAcI || 5.0uL || 0.5uL || 7.0uL || Xba1, 1.0uL || Pst1, 1.0uL || 35.5uL
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|-
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| RFP || 5.0uL || 0.5uL || 4.0uL || Xba1, 1.0uL || Pst1, 1.0uL || 38.5uL
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|}

Revision as of 22:25, 27 July 2008

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1. Spec the plasmid prep products: Place 10 samples into 10 separate plate wells. 11 wells total were used: 1st well was the control so it had 36uL of distilled water and 4uL of elution buffer (since want everything except DNA to be control group), then 2-11 wells had 1-10 plasmid prep products. 2-11 wells had 36uL of distilled water added and 4uL of plasmid prep products. Results:
a. (1) Lac I #1 = [DNA] ng/uL is 45, [DNA] ug/uL is 0.045 HIGH/GOOD
b. (2) Lac I = [DNA] ng/uL is 35, [DNA] ug/uL is 0.035 HIGH/GOOD
c. (3) Lac I = [DNA] ng/uL is 35, [DNA] ug/uL is 0.035 HIGH/GOOD
d. (4) BV:Lac #2 = [DNA] ng/uL is 10, [DNA] ug/uL is 0.001 LOW/POOR
e. (5) BV:Tet #1 = [DNA] ng/uL is 0, [DNA] ug/uL is 0.00 NONE/BAD
f. (6) BV:Tet #2 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005 LOW/POOR
g. (7) BV:Tet/p22 #1 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005 LOW/POOR
h. (8) BV:Tet/p22 #1 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005 LOW/POOR
i. (9) BV:Tet/p22 #2 = [DNA] ng/uL is 335, [DNA] ug/uL is 0.335 HIGH/GOOD
j. (10) BV:Tet/p22 #2 = [DNA] ng/uL is 15, [DNA] ug/uL is 0.015 LOW/POOR
2. Digest Rxn: Using #'s: 1, 2, 6, 9, and 10 from above. Follow the table below:


Parts 10x Buffer BSA DNA RE 1 RE 2 H20
BV:Lac I (1) 5.0uL 0.5uL 22.0uL Spe1, 1.0uL Pst1, 1.0uL 20.5uL
BV:Lac I #2 (2) 5.0uL 0.5uL 28.5uL Spe1, 1.0uL Pst1, 1.0uL 14.0uL
BV:Tet (6) 5.0uL 0.5uL 35.0uL Spe1, 1.0uL Pst1, 1.0uL 7.5uL
BV:Tet/p22 #1 (9) 5.0uL 0.5uL 3.0uL Spe1, 1.0uL Pst1, 1.0uL 39.5uL
BV:Tet/p22 #2 (10) 5.0uL 0.5uL 33.0uL Spe1, 1.0uL Pst1, 1.0uL 9.5uL
p22cII 5.0uL 0.5uL 5.0uL Xba1, 1.0uL Pst1, 1.0uL 37.5uL
LAMBDAcI 5.0uL 0.5uL 7.0uL Xba1, 1.0uL Pst1, 1.0uL 35.5uL
RFP 5.0uL 0.5uL 4.0uL Xba1, 1.0uL Pst1, 1.0uL 38.5uL
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