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Team:Hawaii/Notebook/2008-07-28
From 2008.igem.org
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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Transformation=== :<strong> Margaret</strong> :*Transformation of I14032 into Db3.1 ===Colony PCR=== :<strong> Margar...) |
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:*Verifying the inserts of two I51020 (the base vector) and one colony of pSB1A7 (an insulated vector with high copy #) | :*Verifying the inserts of two I51020 (the base vector) and one colony of pSB1A7 (an insulated vector with high copy #) | ||
| - | ===[[Team:Hawaii/PCR Amplification of pRL1383a|PCR amplification of pRL1383a=== | + | ===[[Team:Hawaii/PCR Amplification of pRL1383a|PCR amplification of pRL1383a]]=== |
:<strong> Margaret</strong> | :<strong> Margaret</strong> | ||
Revision as of 22:04, 28 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Transformation
- Margaret
- Transformation of I14032 into Db3.1
Colony PCR
- Margaret
- Verifying the inserts of two I51020 (the base vector) and one colony of pSB1A7 (an insulated vector with high copy #)
PCR amplification of pRL1383a
- Margaret
- The omega region from pSMC121 and the rep/mob region are giving primer dimers and misamplification respectively. I am trying a gradient PCR from 49°C to 55°C to see if this will fix the problem.
Drylab Work
Primer Design
- Margaret
- I want to design primers for some replication proteins + origin from pSB2K3
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
