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Team:Hawaii/Notebook/2008-07-30
From 2008.igem.org
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:* Digested 5 μl GFP, GFPf, and nir PCR products with EcoRI and SpeI | :* Digested 5 μl GFP, GFPf, and nir PCR products with EcoRI and SpeI | ||
:* Sequentially digested 10 μl pRL1383a and 5 μl J33207 PCR product with BamHI and HindIII | :* Sequentially digested 10 μl pRL1383a and 5 μl J33207 PCR product with BamHI and HindIII | ||
| - | ::* Used Roche enzymes and Roche buffer M (buffer B also works) from SC | + | ::* Used Roche enzymes and Roche buffer M (buffer B also works) from SC |
| - | :* Ran digests on gel | + | :* Ran digests on gel |
| + | ::* pRL1383a and J33207 bands good | ||
| + | ::* GFP, GFPf, and nir bands still too big | ||
| + | |||
| + | ===PCR=== | ||
| + | :<strong>Grace</strong> | ||
| + | |||
| + | :* Redid yesterday's PCR of GFP, GFPf, and nir | ||
===[[Team:Hawaii/PCR Amplification of pRL1383a|PCR amplification of pRL1383a]]=== | ===[[Team:Hawaii/PCR Amplification of pRL1383a|PCR amplification of pRL1383a]]=== | ||
Revision as of 01:39, 31 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Gel Purification
- Grace
- Purified B0030 and B0024 from gel
- B0030 = 21.9 ng/μl
- B0024 = 5.7 ng/μl
Restriction Digest
- Grace
- Digested 5 μl GFP, GFPf, and nir PCR products with EcoRI and SpeI
- Sequentially digested 10 μl pRL1383a and 5 μl J33207 PCR product with BamHI and HindIII
- Used Roche enzymes and Roche buffer M (buffer B also works) from SC
- Ran digests on gel
- pRL1383a and J33207 bands good
- GFP, GFPf, and nir bands still too big
PCR
- Grace
- Redid yesterday's PCR of GFP, GFPf, and nir
PCR amplification of pRL1383a
- Margaret
- ran a gel from yesterday's PCR amplification of oriV, aadA, and rep regions.
Mini-Prep
- Margaret
- Plasmid Prep of [http://partsregistry.org/Part:BBa_I51020 BBa_I51020],[http://partsregistry.org/Part:pSB1A7 pSB1A7], [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
Colony PCR
- Margaret
- verify the insert of the oriT construct and [http://partsregistry.org/Part:BBa_I14032 BBa_I14032]
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
The gel purification kit is almost out of PE. If we need more before our order comes in, there is PE in the Qiaprep Spin Miniprep kit (just remember to add EtOH before use).
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
