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Wisconsin: Lignin Project/19 June 2008
From 2008.igem.org
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Designed new and longer primers <br> | Designed new and longer primers <br> | ||
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| - | + | == Team Sorbitol == | |
| + | |||
| + | Purified the PCR product from yesterday - not enough DNA for a digestion | ||
| + | |||
| + | Re-ran the PCR reaction from yesterday to get more product - however the reaction failed (no bands) | ||
| + | |||
| + | In the meantime designed a digestion reaction to use once we get enough product. | ||
Revision as of 19:54, 31 July 2008
Team Fungus:
Took spec reading of Carbon and Nitrogen-limited cDNA
Worked on Wiki site
Worked on sponsorship
Made overnight cultures of:
BL21 (x2)
pBad33
pMcSG7
pet28a
pet28b
DH1 (x2)
pBad21
Made and autoclaved more LB solution for future use
Designed new and longer primers
Team Sorbitol
Purified the PCR product from yesterday - not enough DNA for a digestion
Re-ran the PCR reaction from yesterday to get more product - however the reaction failed (no bands)
In the meantime designed a digestion reaction to use once we get enough product.
