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Wisconsin: Lignin Project/23 June 2008
From 2008.igem.org
(Difference between revisions)
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Grew overnight culture of pET28a <br> | Grew overnight culture of pET28a <br> | ||
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| - | + | == Team Sorbitol == | |
| + | |||
| + | Decided to use Herculase instead of TAQ in the PCR reactions (Herculase has proofreading ablitity) | ||
| + | |||
| + | Ran a new PCR reaction using the specific volumes designated by the Herculase designers. | ||
| + | |||
| + | No Bands formed. | ||
Revision as of 19:56, 31 July 2008
Team Fungus:
Ran a touchdown PCR of Carbon and Nitrogen-limited cDNA using Herculase II polymerase
Ran PCR products through 0.9% agrose gel at 100V
-Bands present for both at about 1.1kb
Grew overnight culture of pET28a
Team Sorbitol
Decided to use Herculase instead of TAQ in the PCR reactions (Herculase has proofreading ablitity)
Ran a new PCR reaction using the specific volumes designated by the Herculase designers.
No Bands formed.
