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Wisconsin: Lignin Project/26 June 2008
From 2008.igem.org
(Difference between revisions)
(New page: '''Team Fungus:''' <br> Performed ligation of insert and vector with ratios of 0:1, 1:0, 1:3, 3:1, and 1:1 <br> Perform transformation of plasmid into E. coli (BL21) and plated to grow ove...) |
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| - | ''' | + | == Team Sorbitol == |
| + | Found that creating fresh proteinase K solution every time will work better. | ||
| + | |||
| + | Designed and set up a gradiant PCR (using Herculase). | ||
| + | |||
| + | Several successful bands for ''srlD'' were obtained. | ||
| + | |||
| + | The PCR product was purified and a second pcr reaction using the product was performed to maximize our gene sample. | ||
| + | |||
| + | Also designed sequencing primers to test for ''srlD'' in both pBAD30 and pBAD18. | ||
Revision as of 20:03, 31 July 2008
Team Fungus:
Performed ligation of insert and vector with ratios of 0:1, 1:0, 1:3, 3:1, and 1:1
Perform transformation of plasmid into E. coli (BL21) and plated to grow overnight
Team Sorbitol
Found that creating fresh proteinase K solution every time will work better.
Designed and set up a gradiant PCR (using Herculase).
Several successful bands for srlD were obtained.
The PCR product was purified and a second pcr reaction using the product was performed to maximize our gene sample.
Also designed sequencing primers to test for srlD in both pBAD30 and pBAD18.
