Team:Hawaii/Notebook/2008-07-21

From 2008.igem.org

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== Wetlab work ==
== Wetlab work ==
===Colony PCR===
===Colony PCR===
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[[Image:Colony_PCR_7_21.jpg|right|thumb|300px|Colony PCR: verified plasmids pSB1A3, pSB1A2, pSB3K3, and additionally verifying a sample of pSMC121 graciously donated by Dr.C.]]
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:<strong>Margaret</strong>
:<strong>Margaret</strong>
:* Colony PCR of pSB1A2, pSB1A3, pSB3K3
:* Colony PCR of pSB1A2, pSB1A3, pSB3K3
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[[Image:7_21_08_annotated.jpg|right|thumb|300px|Lane 1 contains the 2-log ladder, lane 2: pSB1A3's ccdB insert, lane3: pSB1A2's ccdB insert, lane 4: pSB3K3's ccdB insert, lane 5: positive control, lane 6: negative control, lane 7: pSMC121.]]
 
===PCR amplification of pRL1383a parts===
===PCR amplification of pRL1383a parts===

Revision as of 02:29, 2 August 2008

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Things we did today

Wetlab work

Colony PCR

Colony PCR: verified plasmids pSB1A3, pSB1A2, pSB3K3, and additionally verifying a sample of pSMC121 graciously donated by Dr.C.
Margaret
  • Colony PCR of pSB1A2, pSB1A3, pSB3K3


PCR amplification of pRL1383a parts

Restriction Digest

Krystle
  • Performed 2 restriction digests on nir, gfp, and gfp fusion brick with EcoRI and SpeI
trial 1: digested 5ul of each plasmid prep in a 50ul reaction mixture, ran 20 ul of the digest after 3 hours
trial 2: digested 25ul of each plasmid prep in a 50ul reaction mixture, ran 20 ul of the digest after 2 hours

Drylab Work

Name of Task

Krystle, Margaret
  • We figured out why some of the biobrick extractions did not transform. Some of these parts are on pSB2K3http://partsregistry.org/wiki/index.php?title=Part:pSB2K3 which is a low copy until IPTG is added to the medium in which case it becomes high copy! So next time we make this, add 1mM IPTG to the media.


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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