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Team:Bologna/Notebook
From 2008.igem.org
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== WEEK 1: from 07/21/08 to 07/27/08 == | == WEEK 1: from 07/21/08 to 07/27/08 == | ||
| - | # Enzymatic digestion of: C0051 E\S, LACI* X\P | + | # Enzymatic digestion of: C0051 E\S, LACI* X\P. |
| - | # Gel preparation , electrophoretic run | + | # Gel preparation , electrophoretic run. |
| - | # Amplification of: TETR , PUV, PRECA. | + | # Amplification of: TETR , PUV, PRECA. |
Revision as of 10:41, 5 August 2008
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| HOME | THE PROJECT | THE TEAM | PARTS SUBMITTED TO THE REGISTRY | MODELING | NOTEBOOK |
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Contents |
PROTOCOLS
- Plates preparation
- Amplification
- Transformation
- Inoculation
- Miniprep
- Digestion reaction
- Gel preparation
- Electrophoretic run
- Gel extraction
WEEK 1: from 07/21/08 to 07/27/08
- Enzymatic digestion of: C0051 E\S, LACI* X\P.
- Gel preparation , electrophoretic run.
- Amplification of: TETR , PUV, PRECA.
WEEK 2: from 07/28/08 to 08/03/08
- Transformation of 2008 registry plasmides: I714930 e I714927 (ceppo TOP10)
- Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240
WEEK 3: from 08/04/08 to 08/10/08
