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Team:Hawaii/Notebook/2008-08- 5
From 2008.igem.org
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(→PCR contamination test (cont. from 8/2 and 8/4)) |
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::* Digested nir and J33207 with EcoRI and SpeI | ::* Digested nir and J33207 with EcoRI and SpeI | ||
::* Digested slr1, slr2, pilA with XbaI and PstI | ::* Digested slr1, slr2, pilA with XbaI and PstI | ||
| - | + | :* Ran 20 μl of digests on 2% agarose gel stained with EtBr for 1.5 hours | |
== Drylab Work == | == Drylab Work == | ||
Revision as of 23:51, 5 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Transformation
- Grace
- Transformed 50 μl DB3.1 competent cells with 5 μl ligation reactions of p+r or g+t
- Incubated on ice 17 minutes after adding DNA
PCR contamination test (cont. from 8/2 and 8/4)
- Grace
- Ran an EtBr stained 2.5% gel at 95V for 2 hours
- Why aren't I getting consistent results for any of the lanes?
Construction of p+r and g+t, otra vez
- Grace
- 10 μl PCR reactions for nir, slr1, slr2, pilA, J33207 (in case transformation doesn't work)
- RE digest of PCR products (no gel purification; used 3 μl PCR reactions)
- Digested nir and J33207 with EcoRI and SpeI
- Digested slr1, slr2, pilA with XbaI and PstI
- Ran 20 μl of digests on 2% agarose gel stained with EtBr for 1.5 hours
Drylab Work
Sequencing
- Grace
- Reviewed sequencing results from 7/21, emailed CORE Hawaii with concerns
- Mislabeled samples
- Poor quality reads
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
