Team:BCCS-Bristol/Calendar-Notebook/10 July 2008

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Latest revision as of 08:53, 6 August 2008

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Swimming agar assay with L-aspartate

The swimming agar experiment was repeated. Since the OD₆₀₀ of the bacteria culture for the inoculation was too low, the plates were inoculated the next day. More concentrated aspartic acid solution was made by reacting with NaOH to make aspartic acid salt. A concentration up to 7 % was reached. Also some salt was ordered to save doing this in future. Details under 14 July 2008.

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-Repeated Swimming agar experiment this time with only 0.3% agar + LB without yeast extract at 25, 30 and 37<sup>o</sup>C. incubated overnight
+==Swimming agar assay with L-aspartate==
-made more concentrated aspartic acid solution by reacting with NaOH to make aspartic acid salt, got concentration up to 7%, Also ordered some salt to save doing this in future.
+The swimming agar experiment was repeated. Since the OD<sub>600</sub> of the bacteria culture for the inoculation was too low, the plates were inoculated the next day. More concentrated aspartic acid solution was made by reacting with NaOH to make aspartic acid salt. A concentration up to 7 % was reached.  Also some salt was ordered to save doing this in future. Details under [[Team:BCCS-Bristol/Calendar-Notebook/14 July 2008 | 14 July 2008]].