Alberta NINT/29 July 2008
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(New page: < Back to notebook < Previous entry lab work (SD): Isolated DNA (K102031 and K102032) from 28/07/08 LB + amp...) |
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Ligated K102032/E+P with pSB4A5 (from K102031 /E+P)(Negative control K102031 /E+P). | Ligated K102032/E+P with pSB4A5 (from K102031 /E+P)(Negative control K102031 /E+P). | ||
+ | lab work (JD): | ||
+ | Began creating new input plasmids | ||
+ | Anneal oligos: TAIn_2, TAIn_3, TAIn_6, TAIn_7 | ||
+ | Digest oligos with XhoI and HindIII | ||
+ | Digest K102011 with XhoI and HindIII | ||
+ | Nucleotide removal kit for TAIn_X/XhoI and HindIII | ||
+ | PCR purification kit for K102011/XhoI and HindIII | ||
[[Alberta_NINT/30_July_2008 | Next entry >]] | [[Alberta_NINT/30_July_2008 | Next entry >]] |
Revision as of 18:10, 6 August 2008
lab work (SD):
Isolated DNA (K102031 and K102032) from 28/07/08 LB + amp50 culture tubes using QIAprep spin miniprep protocol, using 5 ml of culture and a minelute spin column, eluting in 15 ul of Buffer EB to maximize the concentration of DNA obtained. Digested K102032 and K102031 with EcoRI + PstI. Ran on 2% agarose gel (110V 50 minutes) and excised the 50 bp fragment from K102032 and the 3 kb fragment from K102031. Extracted DNA from gel usig QIAquick gel extraction protocol. Ligated K102032/E+P with pSB4A5 (from K102031 /E+P)(Negative control K102031 /E+P).
lab work (JD):
Began creating new input plasmids Anneal oligos: TAIn_2, TAIn_3, TAIn_6, TAIn_7 Digest oligos with XhoI and HindIII Digest K102011 with XhoI and HindIII Nucleotide removal kit for TAIn_X/XhoI and HindIII PCR purification kit for K102011/XhoI and HindIII