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Team:Valencia/Notebook/July
From 2008.igem.org
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=== July 2nd === | === July 2nd === | ||
| - | - We prepared our first SD medium. | + | - We prepared our first [[Team:Valencia/Notebook/Protocols#SD Medium | SD medium.]] |
- We calibrated our calorimeters with hot water. | - We calibrated our calorimeters with hot water. | ||
=== July 4th === | === July 4th === | ||
| - | - We prepared some plates for inoculum. | + | - We prepared some plates for inoculum. They were stored a the 28ºC stove. |
=== July 7th === | === July 7th === | ||
| - | - We prepared some inoculum for our experiment the following day. | + | - We prepared some inoculum in Erlenmeyer flasks for our experiment the following day. We kept them overnight in a 30ºC shaking stove. |
=== July 8th === | === July 8th === | ||
| - | - We | + | - The experiment consisted on: <br> |
| + | 300 ml [[Team:Valencia/Notebook/Protocols#SD Medium | SD medium]] <br> | ||
| + | Four strains <br> | ||
| + | We left it overnight, but the air conditioning went off and the room temperature rose. | ||
===July 9th=== | ===July 9th=== | ||
| - | - We characterized culture growth in our calorimeters with just one strain. | + | - We characterized culture growth in our calorimeters with just one strain.<br> |
| + | | ||
===July 10th=== | ===July 10th=== | ||
| - | - Trying to see if there is basal rate of UCP1. | + | - Trying to see if there is basal rate of UCP1. Experiment: |
| + | | ||
===July 11th=== | ===July 11th=== | ||
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===July 15th=== | ===July 15th=== | ||
| - | - We prepared inoculum. | + | - We prepared inoculum in Erlenmeyer flasks. We kept them overnight in a 30ºC shaking stove. |
===July 16th=== | ===July 16th=== | ||
Revision as of 10:05, 7 August 2008
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July 1st
- We prepared some glycerol stock from the four Saccharomyces cerevisiae strains we received from Eduardo Rial.
- We calibrated our calorimeters with hot water.
July 2nd
- We prepared our first SD medium.
- We calibrated our calorimeters with hot water.
July 4th
- We prepared some plates for inoculum. They were stored a the 28ºC stove.
July 7th
- We prepared some inoculum in Erlenmeyer flasks for our experiment the following day. We kept them overnight in a 30ºC shaking stove.
July 8th
- The experiment consisted on:
300 ml SD medium
Four strains
We left it overnight, but the air conditioning went off and the room temperature rose.
July 9th
- We characterized culture growth in our calorimeters with just one strain.
July 10th
- Trying to see if there is basal rate of UCP1. Experiment:
July 11th
- It looks like our Saccharomyces cerevisiae strains have a basal rate of UCP1... We'll have to repeat the experiment to be sure.
July 12th
- We obtained the same results.
July 14th
- The plate is completely dry,the water evaporeted...WHY???? the first unsolved mystery of valencia iGEM 2008.
July 15th
- We prepared inoculum in Erlenmeyer flasks. We kept them overnight in a 30ºC shaking stove.
July 16th
- Trying to find out which is the best moment for activating UCP1...
- We calculated the temperature range in which we expect thermogenine to work within.
July 17th
- Our lab electricity went off during the night. How many experiments will have it ruined?
July 18th
- We prepared lots of plates with our SD medium...Unfortunately, we forgot to add glucose.
- We carried out the pilot experiment. We see weird oscillations.
July 20th
- We stopped the shaking and carried out the experiment again to see whether the oscillations were related to the shaking.
July 21st
- We carried out the pilot experiment again. Weird results, we need to repeat it.
July 22nd
- We repaired our calorimeters. They were the reason for yesterday weird results.
July 23rd
- We repeated the pilot experiment.
July 24th
- Our male group members decided to carried out their own particular experiment involving parafilm, plasticine and teeth filling paste...
July 25th
- Another curious experiment was carried out: one thermocouple was covered with plasticine while the others weren't.
July 26th
- We obtain good results.
July 28th
- One of our calorimeters wasn't working. We repaired it and calibrated once again.
July 29th
- Our cultures haven't grown enough for the experiment. Temperature and medium was all right, we'll have to wait...
July 30th
- We carried out the pilot experiment. Weird results...
