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Team:ESBS-Strasbourg/6 August 2008
From 2008.igem.org
(Difference between revisions)
(New page: < back == DryLab == === Modeling === == WetLab == ''Katja'' *PCR confirms the identy of the Gal4O, LacIO, LexAO, cIO, tetO BioBricks (Which are in the...) |
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*Digestion with SpeI and PstI of the LacIO, LexAO, cIO and the tetO vectors -> back vectors | *Digestion with SpeI and PstI of the LacIO, LexAO, cIO and the tetO vectors -> back vectors | ||
*Ethanolic precipitation to get ride of the small fragments | *Ethanolic precipitation to get ride of the small fragments | ||
| + | |||
| + | ''Paul''<br> | ||
| + | |||
| + | -Digestion E+S of c1/lacI/lexA/tetR/EYFP/adh1-prom -> get insert <br> | ||
| + | -Digestion X+P adh1-ter -> get insert <br> | ||
| + | -Digestion E+X linker/Kozak -> get vector <br> | ||
| + | -Digestion S+P NLS -> get vector <br> | ||
| + | |||
| + | Assembly we want to perform:<br> | ||
| + | c1+linker <br> | ||
| + | lacI+linker <br> | ||
| + | lexA+linker <br> | ||
| + | tetR+linker <br> | ||
| + | NLS+adh1term <br> | ||
| + | EYFP+linker <br> | ||
| + | adh1P+kozak <br> | ||
== General == | == General == | ||
== Quote of the day == | == Quote of the day == | ||
Latest revision as of 10:00, 8 August 2008
Contents |
DryLab
Modeling
WetLab
Katja
- PCR confirms the identy of the Gal4O, LacIO, LexAO, cIO, tetO BioBricks (Which are in the pSB1A2 backbone and in the Silver-standard)
- Miniprep of the overnight cultures
- Digestion with SpeI and PstI of the LacIO, LexAO, cIO and the tetO vectors -> back vectors
- Ethanolic precipitation to get ride of the small fragments
Paul
-Digestion E+S of c1/lacI/lexA/tetR/EYFP/adh1-prom -> get insert
-Digestion X+P adh1-ter -> get insert
-Digestion E+X linker/Kozak -> get vector
-Digestion S+P NLS -> get vector
Assembly we want to perform:
c1+linker
lacI+linker
lexA+linker
tetR+linker
NLS+adh1term
EYFP+linker
adh1P+kozak
