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Team:Hawaii/Notebook/2008-08- 8
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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Restreaked nir+rbs and I14032+rbs constructs=== :<strong> Grace</strong> ===Prep for sequencing=== [[Image:080808PCR.j...)
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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Restreaked nir+rbs and I14032+rbs constructs=== :<strong> Grace</strong> ===Prep for sequencing=== [[Image:080808PCR.j...)
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Revision as of 10:44, 8 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Restreaked nir+rbs and I14032+rbs constructs
- Grace
Prep for sequencing
- Grace
- 25 μl PCR reactions of nir+rbs, I14032+rbs, slr1, slr2, BB-pRL1383a
- Colony PCRs seem to indicate success
- 25 μl PCR reactions of GFP+tt, GFPf+tt, J33207+tt
- Colony PCR indicates no ligation. Picked new colony, sequence to confirm failure.
- Gel purified all PCR rxns (we still have a problem with contaminant DNA/shadow bands) and desired bands were extracted from gel
- Determined DNA concentrations via nanodrop spectrometer
- Prepared samples and sent to CORE Hawaii for sequencing
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
