Wisconsin: Lignin Project/24 June 2008

From 2008.igem.org

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|'''Team Sorbitol:'''
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Attempted to isolate genomic DNA from E. coli MG1655 using the Weibel lab protocols.<br>
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Using this isolated DNA ran a pcr reaction as before and got one successful band at 700bp.<br>
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We purified and stored this band at -20.<br>
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'''Team Fungus:''' <br>
'''Team Fungus:''' <br>
Ran leftovers from yesterday's PCR in 1% agrose gel<br>
Ran leftovers from yesterday's PCR in 1% agrose gel<br>
-correct sized bands visible in UV light <br>
-correct sized bands visible in UV light <br>
Used QIAquick gel extraction microcentrifuge kit and QIAquick PCR purification to get purified product<br>
Used QIAquick gel extraction microcentrifuge kit and QIAquick PCR purification to get purified product<br>
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<br>
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Used QIAprep miniprep kit to purify pET28a vectors from liquid LB + Kanamycin media that had incubated overnight<br>
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Used QIAprep miniprep kit to purify pET28a vectors from liquid LB + Kanamycin media that had incubated overnight
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<br>
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Ran another touchdown PCR of both Carbon and Nitrogen limited cDNA using Herculase II polymerase <br>
Ran another touchdown PCR of both Carbon and Nitrogen limited cDNA using Herculase II polymerase <br>
Used QIAquick PCR purification kit to purify PCR product <br>
Used QIAquick PCR purification kit to purify PCR product <br>
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|}
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== Team Sorbitol ==
+
-
Attempted to isolate genomic DNA from E. coli MG1655 using the Weibel lab protocols.
+
-
 
+
-
Using this isolated DNA ran a pcr reaction as before and got one successful band at 700bp.
+
-
 
+
-
We purified and stored this band at -20.
+

Revision as of 15:23, 8 August 2008

Igemwibanner.gif
Team Sorbitol:

Attempted to isolate genomic DNA from E. coli MG1655 using the Weibel lab protocols.
Using this isolated DNA ran a pcr reaction as before and got one successful band at 700bp.
We purified and stored this band at -20.

Team Fungus:
Ran leftovers from yesterday's PCR in 1% agrose gel
-correct sized bands visible in UV light
Used QIAquick gel extraction microcentrifuge kit and QIAquick PCR purification to get purified product
Used QIAprep miniprep kit to purify pET28a vectors from liquid LB + Kanamycin media that had incubated overnight
Ran another touchdown PCR of both Carbon and Nitrogen limited cDNA using Herculase II polymerase
Used QIAquick PCR purification kit to purify PCR product

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