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Wisconsin: Lignin Project/26 June 2008
From 2008.igem.org
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| + | |'''Team Sorbitol:''' | ||
Found that creating fresh proteinase K solution every time will work better. | Found that creating fresh proteinase K solution every time will work better. | ||
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Also designed sequencing primers to test for ''srlD'' in both pBAD30 and pBAD18. | Also designed sequencing primers to test for ''srlD'' in both pBAD30 and pBAD18. | ||
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| + | '''Team Fungus:''' <br> | ||
| + | Performed ligation of insert and vector with ratios of 0:1, 1:0, 1:3, 3:1, and 1:1 <br> | ||
| + | Perform transformation of plasmid into E. coli (BL21) and plated to grow overnight | ||
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Revision as of 15:25, 8 August 2008
| Team Sorbitol:
Found that creating fresh proteinase K solution every time will work better. Designed and set up a gradiant PCR (using Herculase). Several successful bands for srlD were obtained. The PCR product was purified and a second pcr reaction using the product was performed to maximize our gene sample. Also designed sequencing primers to test for srlD in both pBAD30 and pBAD18. Team Fungus: |
