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Wisconsin: Lignin Project/7 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: == Team Sorbitol == The 10 colonies were harvested with a Qiagen Miniprep Kit and the DNA was digested with Xba1 and HindIII for 1 hour at 37C. The digestion was then run on an agarose g...) |
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| - | + | |'''Team Sorbitol:'''<br> | |
| - | + | The 10 colonies were harvested with a Qiagen Miniprep Kit and the DNA was digested with Xba1 and HindIII for 1 hour at 37C.<br> | |
| - | The 10 colonies were harvested with a Qiagen Miniprep Kit and the DNA was digested with Xba1 and HindIII for 1 hour at 37C. | + | The digestion was then run on an agarose gel along with controls and undigested vector.<br> |
| - | + | We found that each colony contained accurate bands indicating pBAD30 as well as ''srlD''.<br> | |
| - | The digestion was then run on an agarose gel along with controls and undigested vector. | + | Plated these accurate colonies and stored them all in a fridge at 4C as well as grew some colonies overnight.<br> |
| - | + | |} | |
| - | We found that each colony contained accurate bands indicating pBAD30 as well as ''srlD''. | + | |
| - | + | ||
| - | Plated these accurate colonies and stored them all in a fridge at 4C as well as grew some colonies overnight. | + | |
Revision as of 15:39, 8 August 2008
|Team Sorbitol:
The 10 colonies were harvested with a Qiagen Miniprep Kit and the DNA was digested with Xba1 and HindIII for 1 hour at 37C.
The digestion was then run on an agarose gel along with controls and undigested vector.
We found that each colony contained accurate bands indicating pBAD30 as well as srlD.
Plated these accurate colonies and stored them all in a fridge at 4C as well as grew some colonies overnight.
|}
