Wisconsin: Lignin Project/18 July 2008

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(Difference between revisions)
(New page: == Team Sorbitol == Froze down 3 400ul samples of RL257, MG1655, and JW3890 each containing pBAD30-srlD. They were then stored at -80. Primers were then designed and ordered to insert '...)
 
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== Team Sorbitol ==
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Froze down 3 400ul samples of RL257, MG1655, and JW3890 each containing pBAD30-srlD.
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<div style="width:800px; height:147px; border:0px; margin:0px;">[[Image:Igemwibanner.gif]]</div>
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They were then stored at -80.
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{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
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|'''Team Sorbitol:'''
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Froze down 3 400ul samples of RL257, MG1655, and JW3890 each containing pBAD30-srlD.<br>
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They were then stored at -80.<br>
Primers were then designed and ordered to insert ''srlD'' into the pET28a vector which will provide a His tag that can be used to purify the enzyme.
Primers were then designed and ordered to insert ''srlD'' into the pET28a vector which will provide a His tag that can be used to purify the enzyme.
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Latest revision as of 15:45, 8 August 2008

Igemwibanner.gif
Team Sorbitol:

Froze down 3 400ul samples of RL257, MG1655, and JW3890 each containing pBAD30-srlD.
They were then stored at -80.
Primers were then designed and ordered to insert srlD into the pET28a vector which will provide a His tag that can be used to purify the enzyme.