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Team:Hawaii/Notebook/2008-08- 8
From 2008.igem.org
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(→Restreaked nir+rbs and I14032+rbs constructs) |
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:* 25 μl PCR reactions of GFP+tt, GFPf+tt, J33207+tt | :* 25 μl PCR reactions of GFP+tt, GFPf+tt, J33207+tt | ||
::* Colony PCR indicates no ligation. Picked new colony, sequence to confirm failure. | ::* Colony PCR indicates no ligation. Picked new colony, sequence to confirm failure. | ||
| + | :* PCR of nir, B0015, B0030, B0034 for resequencing (bad reads last time) | ||
:* Gel purified all PCR rxns (we still have a problem with contaminant DNA/shadow bands) and desired bands were extracted from gel | :* Gel purified all PCR rxns (we still have a problem with contaminant DNA/shadow bands) and desired bands were extracted from gel | ||
::* 2% agarose gel ran at 60v for 2 hours | ::* 2% agarose gel ran at 60v for 2 hours | ||
Revision as of 20:16, 8 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Restreaked nir+rbs and I14032+rbs constructs
- Grace
Plasmid prep (finished up)
- Grace
- Resuspended plasmid preps in 50 μl TE buffer
- Determined DNA concentrations of plasmids
| Plasmid | DNA Concentration |
|---|---|
| nir | 496.3 ng/μl |
| GFPf | 484.8 ng/μl |
| BB-pRL1383a | 508.0 ng/&mul |
Prep for sequencing
- Grace
- 25 μl PCR reactions of nir+rbs, I14032+rbs, slr1, slr2, BB-pRL1383a
- Colony PCRs seem to indicate success
- 25 μl PCR reactions of GFP+tt, GFPf+tt, J33207+tt
- Colony PCR indicates no ligation. Picked new colony, sequence to confirm failure.
- PCR of nir, B0015, B0030, B0034 for resequencing (bad reads last time)
- Gel purified all PCR rxns (we still have a problem with contaminant DNA/shadow bands) and desired bands were extracted from gel
- 2% agarose gel ran at 60v for 2 hours
- Determined DNA concentrations via nanodrop spectrometer
- Prepared samples and sent to CORE Hawaii for sequencing
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
