Team:TU Munchen/Projects

From 2008.igem.org

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==Detection of Mechanical Stress==
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==Mechanical Stress Detection==
* '''Goal:''' Make E. coli respond to mechanical stress.
* '''Goal:''' Make E. coli respond to mechanical stress.
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* '''Questions:'''
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** How does [http://en.wikipedia.org/wiki/Mechanotransduction mechanotransduction] work in E. coli?
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* '''Notes:'''
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* '''References:'''
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** [http://www.childrenshospital.org/research/ingber/PDF/2002/STKE-Alenghat.pdf]
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** [http://www.proteinscience.org/cgi/content/abstract/8/10/1915 Structure and Function of the Bacterial Mechanosensitive Channel of Large Conductance]

Revision as of 14:26, 29 May 2008

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Contents

Product Transport in E. Coli (E. Coli Filter)

  • Goal: Make E. coli absorb some product.
  • Questions:
    • What products do we want E. coli to absorb?
    • How should E. coli absorb the products?
  • Notes:
    • Someone should research [http://en.wikipedia.org/wiki/Bioremediation bioremediation] to determine what products cause problems.
    • We should understand transport across the cell envelope of [http://en.wikipedia.org/wiki/Gram-negative Gram-negative bacteria].
    • E. coli do transport iron across their cell envelope. A great review is [http://www.sciencemag.org/cgi/reprint/295/5560/1658.pdf Close Before Opening].
    • A starting point for other metals is [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T1W-4JTJ9T5-1&_user=616147&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000032324&_version=1&_urlVersion=0&_userid=616147&md5=a755ffdf33ea1c8d5f687b7385e3f250 Structural Analysis and Classification of Native Proteins from E. Coli Commonly Co-Purified by Immobilised Metal Affinity Chromatography].


Chemotaxis Control (E. Coli Filter and E. Coli Rugby)

  • Goal: Make E. coli follow/flee some chemical attractant/repellent.
  • Questions:
    • How does the chemotactic system work?
  • Notes:
    • Read [http://pubs.acs.org/cgi-bin/sample.cgi/jacsat/2007/129/i21/pdf/ja0692480.pdf Guiding Bacteria with Small Molecules and RNA].


Mechanical Stress Detection

  • Goal: Make E. coli respond to mechanical stress.
  • Questions:
    • How does [http://en.wikipedia.org/wiki/Mechanotransduction mechanotransduction] work in E. coli?
  • Notes:
  • References:
    • [http://www.childrenshospital.org/research/ingber/PDF/2002/STKE-Alenghat.pdf]
    • [http://www.proteinscience.org/cgi/content/abstract/8/10/1915 Structure and Function of the Bacterial Mechanosensitive Channel of Large Conductance]


LPS Detection

  • Goal: Make E. coli detect LPS inflammation.


E. Coli Digestion

  • Goal:
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