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Alberta NINT/11 August 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/ | + | [[Alberta_NINT/6_August_2008 | < Previous entry]] |
lab work (SD): | lab work (SD): | ||
Ligation of TA2, TA3, TA6 and TA7 into vector K102031. | Ligation of TA2, TA3, TA6 and TA7 into vector K102031. | ||
Transformed XL1-B cells with the ligations. | Transformed XL1-B cells with the ligations. | ||
| + | |||
| + | lab work (JD): | ||
| + | Annealed TAIn_2, TAIn_3, TAIn_6 and TAIn_7 | ||
| + | Nucleotide Removal | ||
| + | Restriction Digest (2hrs) with XhoI + HindIII | ||
| + | Ran on gel 2%, 80V for 2 hours | ||
| + | [[Image:UVP00336.jpg]] | ||
| + | |||
[[Alberta_NINT/12_August_2008 | Next entry >]] | [[Alberta_NINT/12_August_2008 | Next entry >]] | ||
Revision as of 20:17, 12 August 2008
lab work (SD):
Ligation of TA2, TA3, TA6 and TA7 into vector K102031.
Transformed XL1-B cells with the ligations.
lab work (JD):
Annealed TAIn_2, TAIn_3, TAIn_6 and TAIn_7
Nucleotide Removal
Restriction Digest (2hrs) with XhoI + HindIII
Ran on gel 2%, 80V for 2 hours
