Team:Hawaii/Notebook/2008-08-12

From 2008.igem.org

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Revision as of 21:42, 12 August 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Test of lab's RE (cont.)

Grace
EtBr stained 1.2% agarose gel ran at 72V for 1.5 hours. Thirty microliters of RE digest reaction were loaded into each well.
  • Ran RE digests from yesterday on a 1.2% agarose gel

Verified transformants

Grace
EtBr stained 2% agarose gel ran at 95V for 1 hour. Five microliters of PCR reaction were loaded into each well.
Construct Colony forming units
GFP+B0015(tt) 1
GFPf+B0015(tt) 52
slr1+GFPf 56
pilA+GFPf 43
  • Colony PCR of transformants to check for correct insert
  • Ligated slr, pilA with GFPf (ES digested) instead of GFPf (XP digested). Still got colonies. Hm...

3A assembly

Grace
  • Ligated p+r with g or s onto pSB1A2
  • pnir+rbs with GFP, slr1, pilA
  • plac+rbs with GFP, slr, pilA
  • Transformed into DH5α

Drylab Work

Name of Task

name of person/people who performed the task
  • Summary of task and what was done. Link to experiment for detailed notes if necessary.
  • e.g. read through papers, worked on proposal, etc.


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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