Team:Hawaii/Notebook/2008-08-13

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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of Transformants=== [[Image:081308colonyPCR.jpg|right|thumb|300px|EtBr stained 2% agarose gel ran at 95V f...)
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Revision as of 05:40, 13 August 2008

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Notebook (t) Meetings (t)

Things we did today

Wetlab work

Verification of Transformants

EtBr stained 2% agarose gel ran at 95V for 1 hour. Five microliters of PCR reaction were loaded into each well.
Grace
Construct Colony forming units
slr1 + GFPf
pilA + GFPf
nir+rbs + GFP
nir+rbs + slr1
nir+rbs + pilA
plac+rbs + GFP
plac+rbs + slr1
plac+rbs + pilA
  • Colony PCR of transformants
  • Ran on 2% agarose gel at 95V for 1 hour

Drylab Work

Sequencing

Grace
  • Checked sequencing results returned from CORE Hawaii


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]