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Alberta NINT/29 May 2008
From 2008.igem.org
(Difference between revisions)
(New page: lab work (SD): Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B) (plates from 28/05/09). DNA was run on 2% agarose gel. All 9 samples were identical on th...) |
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lab work (SD): Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B) (plates from 28/05/09). DNA was run on 2% agarose gel. All 9 samples were identical on the gel. K102002.2, K102002.4, K102002.5, K102002.8, K102004.1, K102004.3, K102004.4, and K102004.5 were selected at random and inoculated into LB + amp50 culture tubes and incubated overnight at 37 C. | lab work (SD): Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B) (plates from 28/05/09). DNA was run on 2% agarose gel. All 9 samples were identical on the gel. K102002.2, K102002.4, K102002.5, K102002.8, K102004.1, K102004.3, K102004.4, and K102004.5 were selected at random and inoculated into LB + amp50 culture tubes and incubated overnight at 37 C. | ||
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| + | lab work (JD): Gel purified R0011 band from gel electrophoresis. Carried out quantitative analysis and carried out ligation to create K102001 (R0011+T/A1), transformed XL1-B cells with K102001. Prepared R0011 for sequencing and dropped it off. | ||
Revision as of 22:24, 30 May 2008
lab work (SD): Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B) (plates from 28/05/09). DNA was run on 2% agarose gel. All 9 samples were identical on the gel. K102002.2, K102002.4, K102002.5, K102002.8, K102004.1, K102004.3, K102004.4, and K102004.5 were selected at random and inoculated into LB + amp50 culture tubes and incubated overnight at 37 C.
lab work (JD): Gel purified R0011 band from gel electrophoresis. Carried out quantitative analysis and carried out ligation to create K102001 (R0011+T/A1), transformed XL1-B cells with K102001. Prepared R0011 for sequencing and dropped it off.
