"
Team:Hawaii/Notebook/2008-08-15
From 2008.igem.org
(Difference between revisions)
(→Name of Task) |
(→Reconstruction of BB-pRL1383a) |
||
| Line 9: | Line 9: | ||
:* PCR amplification of J33207 | :* PCR amplification of J33207 | ||
::* ExoSAP'd PCR product | ::* ExoSAP'd PCR product | ||
| - | :* RE digested of J33207 and BB-pRL1383a (w/ GFP) | + | :* RE digested of J33207 and BB-pRL1383a (w/ GFP) with EcoRI and PstI |
:* Ran digests on a 1% agarose gel at 72V for 1.5 hours. | :* Ran digests on a 1% agarose gel at 72V for 1.5 hours. | ||
::* Ladder did not run well. Bands not resolving well. | ::* Ladder did not run well. Bands not resolving well. | ||
| - | :* Redid PCR and RE digests | + | :* Redid PCR and RE digests with XbaI and PstI |
== Drylab Work == | == Drylab Work == | ||
Revision as of 01:54, 16 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Reconstruction of BB-pRL1383a
- Grace
- PCR amplification of J33207
- ExoSAP'd PCR product
- RE digested of J33207 and BB-pRL1383a (w/ GFP) with EcoRI and PstI
- Ran digests on a 1% agarose gel at 72V for 1.5 hours.
- Ladder did not run well. Bands not resolving well.
- Redid PCR and RE digests with XbaI and PstI
Drylab Work
Editing team website
- Grace
Discussion
- DH5α does not carry lacIq; therefore, IPTG is not neccessary to induce the lac promoter.
- IPTG is necessary for induction in DB3.1 cells.
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
