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Team:Hawaii/Notebook/2008-05-30
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(New page: {{Team:Hawaii/Header}} == Plated ''E. coli'' == '''Grace & Norman''' Plated ''E. coli'' strain DH5-alpha on LB plates made yesterday :*one plate was plated using the hockey puck method f...)
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(New page: {{Team:Hawaii/Header}} == Plated ''E. coli'' == '''Grace & Norman''' Plated ''E. coli'' strain DH5-alpha on LB plates made yesterday :*one plate was plated using the hockey puck method f...)
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Revision as of 02:09, 31 May 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Plated E. coli
Grace & Norman
Plated E. coli strain DH5-alpha on LB plates made yesterday
- one plate was plated using the hockey puck method for maximum growth
- one plate was plated using the 4-way streak method to isolate colonies
Plates were placed in a 37C incubator for 5 hours then transfered to a 4C incubator for storage.
Preparation for making competant cells
Grace, Margaret, & Norman
Made SOB media for growing E. coli DH5-alpha
Made 20mM glucose solution
Discussion
- Dr. Presting: "Wow, I didn't know three people could fit in this room."
- M: "Well, not maybe three of you."
- Dr. Presting: "Are you calling me fat?"
- M: "No, no. You're tall, dark, and handsome!"
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
