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Team:Hawaii/Notebook/2008-08-16
From 2008.igem.org
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[[Image:081608REdigest.jpg|right|thumb|200px|EtBr stained 1% agarose gel ran at 60V for 2 hours. Thirty microliters of RE digested DNA were loaded into each well.]] | [[Image:081608REdigest.jpg|right|thumb|200px|EtBr stained 1% agarose gel ran at 60V for 2 hours. Thirty microliters of RE digested DNA were loaded into each well.]] | ||
:* Ran digests on a 1% agarose gel | :* Ran digests on a 1% agarose gel | ||
| + | :* Extracted bands from gel | ||
| + | :* Ligated: | ||
| + | ::* 1 μl XbaI/PstI digested BB-pRL1383a with 3 μl XbaI/PstI digested J33207 | ||
| + | ::* 1 μl XbaI/PstI digested BB-pRL1383a to itself (negative control) | ||
| + | :* Transformed into DH5α | ||
| + | ::* Used 5 μl ligation reaction to transform | ||
| + | ::* Plated on LB+sp<sub>100</sub>+X-gal and LB+sp<sub>100</sub>+sm<sub>50</sub>+IPTG+X-gal | ||
| + | :::* To verify if IPTG is needed for lacZ expression | ||
= Discussion = | = Discussion = | ||
Revision as of 00:44, 17 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Reconstruction of BB-pRL1383a (cont.)
- Grace
- Ran digests on a 1% agarose gel
- Extracted bands from gel
- Ligated:
- 1 μl XbaI/PstI digested BB-pRL1383a with 3 μl XbaI/PstI digested J33207
- 1 μl XbaI/PstI digested BB-pRL1383a to itself (negative control)
- Transformed into DH5α
- Used 5 μl ligation reaction to transform
- Plated on LB+sp100+X-gal and LB+sp100+sm50+IPTG+X-gal
- To verify if IPTG is needed for lacZ expression
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
