Team:Hawaii/Notebook/2008-08-17

From 2008.igem.org

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===Verification of transformants===
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:<strong> Grace</strong>
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[[Image:081708colonyPCR.jpg|right|thumb|250px|EtBr stained 2% agarose gel ran at 95V for 1 hour. Five microliters of PCR reaction were loaded into each well.]]
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= Discussion =
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Revision as of 01:47, 17 August 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Verification of transformants

Grace
EtBr stained 2% agarose gel ran at 95V for 1 hour. Five microliters of PCR reaction were loaded into each well.
DNA Total colony forming units Blue colonies
BB-pRL1383a + J33207 (plated on IPTG + X-gal)
BB-pRL1383a + J33207 (plated on X-gal)
BB-pRL1383a (negative control)
no DNA (negative control)
  • Colony PCR to verify insert

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]