Team:Hawaii/Notebook/2008-08-17
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(Difference between revisions)
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+ | [[Image:081708colonyPCR.jpg|right|thumb|250px|EtBr stained 2% agarose gel ran at 95V for 1 hour. Five microliters of PCR reaction were loaded into each well.]] | ||
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Revision as of 01:47, 17 August 2008
Projects | Events | Resources | ||
---|---|---|---|---|
Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
DNA | Total colony forming units | Blue colonies |
---|---|---|
BB-pRL1383a + J33207 (plated on IPTG + X-gal) | ||
BB-pRL1383a + J33207 (plated on X-gal) | ||
BB-pRL1383a (negative control) | ||
no DNA (negative control) |
- Colony PCR to verify insert
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]