"
Team:Valencia/Notebook/Protocols
From 2008.igem.org
(Difference between revisions)
(→SD Medium) |
|||
| Line 32: | Line 32: | ||
{| | {| | ||
|Yeast Nitrogen Base | |Yeast Nitrogen Base | ||
| + | | | ||
|0.67% | |0.67% | ||
|- | |- | ||
| Line 49: | Line 50: | ||
|40 mg/l | |40 mg/l | ||
|} | |} | ||
| - | |||
== SP liquid medium == | == SP liquid medium == | ||
Revision as of 12:40, 19 August 2008
Glycerol Stock
2 ml Eppendorf containing:
| Sterile Glycerol (stock 50 %) | 1ml |
| Half culture plate |
SD Medium
| Yeast Nitrogen Base | 0.67% | |
| Glucose | 2% | |
| Agar | 2% | |
| Casamino acids | 0.1% | |
| Tryptophan | 20 mg/l | |
| Adenine | 40 mg/l |
SP liquid medium
Yeast Nitrogen Base 0.67%
Potassium phosohate 0.1%
Ammonium sulfate 0.12%
Glucose 0.05%
Lactic acid 2%
Casamino acids 0.1%
Tryptophan 20 mg/l
Adenine 40 mg/l
YPKAc Medium
Yeast extract 1%
Peptone 2%
Potassium acetate 2%
Tryptophan 20 mg/l
Adenine 40 mg/l
