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Team:Valencia/Notebook/Protocols
From 2008.igem.org
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Revision as of 12:44, 19 August 2008
Glycerol Stock
2 ml Eppendorf containing:
| Sterile Glycerol (stock 50 %) | 1ml |
| Half volume of a culture plate |
SD Medium
| Yeast Nitrogen Base | 0.67% |
| Glucose | 2% |
| Agar | 2% |
| Casamino acids | 0.1% |
| Tryptophan | 20 mg/l |
| Adenine | 40 mg/l |
SP liquid medium
Yeast Nitrogen Base 0.67%
Potassium phosohate 0.1%
Ammonium sulfate 0.12%
Glucose 0.05%
Lactic acid 2%
Casamino acids 0.1%
Tryptophan 20 mg/l
Adenine 40 mg/l
YPKAc Medium
Yeast extract 1%
Peptone 2%
Potassium acetate 2%
Tryptophan 20 mg/l
Adenine 40 mg/l
