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Team:Hawaii/Notebook/2008-08-19
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Normanwang (Talk | contribs) (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Name of Task=== :<strong> name of person/people who performed the task</strong> :* Summary of task and what was done. ...) |
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= Things we did today = | = Things we did today = | ||
== Wetlab work == | == Wetlab work == | ||
| - | === | + | ===RE test=== |
| - | :<strong> | + | :<strong>Krystle</strong> |
| - | :* | + | :* Checked to see if RE are still active by cutting R0010 with EcoRI, XbaI, SpeI, and PstI individually |
| - | :* | + | :* Ran on 2% agarose gel |
| + | ::* R0010 was probably from a plasmid prep where cells did not lyse, i.e. no DNA in prep | ||
| + | ::* Oops, forgot to run uncut plasmid as control | ||
| + | :* Set up new RE digest (w/ C0012) overnight to check enzymatic activity | ||
| + | ::* Also checked EcoRI enzyme in TKW box of lab freezer | ||
| + | ===Verification of transformants=== | ||
| + | :<strong> Grace</strong> | ||
| + | [[Image:081908.jpg|right|thumb|250px|EtBr stained 2% agarose gel ran at 95V for 1 hour. Thirty microliters of RE digest and ten microliters of PCR reaction were loaded into each lane.]] | ||
| + | :* Plasmid prep of BB-pRL1383a+J33207 plasmid to verify replacement of GFP with J33207 (lac device) | ||
| + | :* PCR of BB-pRL1383a+J33207 plasmid preps | ||
| + | ::* Negative = water | ||
| + | ::* Positive = BB-pRL1383a | ||
| + | :* Ran on 2% agarose gel | ||
== Drylab Work == | == Drylab Work == | ||
Revision as of 08:27, 20 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
RE test
- Krystle
- Checked to see if RE are still active by cutting R0010 with EcoRI, XbaI, SpeI, and PstI individually
- Ran on 2% agarose gel
- R0010 was probably from a plasmid prep where cells did not lyse, i.e. no DNA in prep
- Oops, forgot to run uncut plasmid as control
- Set up new RE digest (w/ C0012) overnight to check enzymatic activity
- Also checked EcoRI enzyme in TKW box of lab freezer
Verification of transformants
- Grace
- Plasmid prep of BB-pRL1383a+J33207 plasmid to verify replacement of GFP with J33207 (lac device)
- PCR of BB-pRL1383a+J33207 plasmid preps
- Negative = water
- Positive = BB-pRL1383a
- Ran on 2% agarose gel
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
What?!? Grrr...... - Krystle
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
