Revision as of 08:28, 20 August 2008

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Things we did today

Krystle

Checked to see if RE are still active by cutting R0010 with EcoRI, XbaI, SpeI, and PstI individually
Ran on 2% agarose gel

R0010 was probably from a plasmid prep where cells did not lyse, i.e. no DNA in prep
Oops, forgot to run uncut plasmid as control

Grace

EtBr stained 2% agarose gel ran at 95V for 1 hour. Thirty microliters of RE digest and ten microliters of PCR reaction were loaded into each lane.

Plasmid prep of BB-pRL1383a+J33207 plasmid to verify replacement of GFP with J33207 (lac device)
PCR of BB-pRL1383a+J33207 plasmid preps

Krystle

What?!? Grrr...... - Krystle

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 23: / Line 23: @@
 == Drylab Work ==
-===Name of Task===
+===Updated project descriptions on wiki===
-:<strong> name of person/people who performed the task</strong>
+:<strong> Krystle</strong>
-:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
-:* e.g. read through papers, worked on proposal, etc.
 = Discussion =