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Edinburgh/25 June 2008
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Revision as of 11:04, 20 August 2008
Week 2
Wednesday 25 June 08
- Primers arrived. Made up 500μM stock solutions in EB and 10μM working solutions (f and r together) in water. Performed PCR with KOD, annealing at 55°C and extending for 38s (expected sizes are: dxs 1862 + prefix and suffix; appY 749 + prefix and suffix; glgC 1295 with no prefix or suffix (except the extra TAA). Result: all looked good, nice pure bands.
Gel 1: markers, empty lane, dxs, appY, glgC, appY (repeat), glgC (repeat), markers.
