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Edinburgh/2 July 2008
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Revision as of 11:07, 20 August 2008
Week 3
Wednesday 2 July 08
- Digests of M10 and M11 with EcoRI, to determine orientation, were not very clear (Gel 7). Probably simpler just to sequence them, since we will need to check that the ends are intact in any case. Ordered mutagenic primers to remove the EcoRI sites:
primer glgcm1f: tgttgaaaaacctgctaaccc
primer glgcm1r: aattcgataattttatcgttctc
primer glgcm2f: ctcattctgcaacattgattcc
primer glgcm2r: ttcacgcgaacgcgcgag
