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Edinburgh/10 July 2008
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Revision as of 11:11, 20 August 2008
Week 4
Thursday 10 July 08
- Subitted M15 (possible pSB1A2+appY) for sequencing with primers pSB1A2insf2 and pSB1A2insr2.
- Minipreps M19 to M24 of possible glgC mutants with EcoRI site 2 removed. EcoRI digests were run on Gel 11. The insert bands are a bit fuzzy, but it looks as though M19, M21 and M22 may have lost the site, and M20, M23 and M24 may still have it.
- PCR P11 using ligation L8 as template to generate a fusion product of rbs+dxs. Run on Gel 11, last lane. No product visible.
- Digested P8 and P9 (crtB and crtI-mut PCR products) with EcoRI/SpeI together with Edinbrick1, purified and ligated (ligations L9 and L10) at 16°C overnight.
