Edinburgh/12 July 2008

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Edinburgh iGEM 2008

 

Week 4

Saturday 12 July 08

  • Result of transformation: crtB 100μl, 22 white and 10 blue; 900μl, too numerous to count, about two thirds white; a few blue showed signs of phage-like lysis. For crtI mutant, 100μl, 4 white and 2 blue; 900μl, 143 white and 25 blue. White crtB colonies were patched to Plate 35, crtI to Plate 36.
  • Set up overnight cultures of possible rbs+dxs clones from plate 34 for minipreps.