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Edinburgh/13 July 2008
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Revision as of 11:11, 20 August 2008
Week 4
Sunday 13 July 08
- Signs of growth on Cellulomonas plates 28 and 29.
- Minipreps M25 to M30 of possible rbs+dxs clones from plate 34.
- PCR P12 to obtain rbs+dxs fusion product in case minipreps didn't work. Template was ligation L11, and primers were rbs2clonf1 and pSB1A2insr1. Annealing was 58°C, extension 40s using KOD.
