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Edinburgh/22 July 2008
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Revision as of 11:18, 20 August 2008
Week 6
Tuesday 22 July 08
- Positive control PCR with universal primers fd1 and rd1 to amplify 16S rRNA gene rrnB from C. fimi (P26); PCR products run on Gel 21 - failed. (AM)
- Made overnight culture of C. fimi from plate 37 for chromosomal DNA purification tomorrow. (AM)
- M36 (crtB) and M42 (crtI) double digested, ligated with rbs to make L17 (M36) and L18 (M42). (YAN & OG)
- L15 (rbs+dxs ligation to Edinbrick1) and L16 (rbs+appY ligation to Edinbrick1) run on gel, the result showed the ligations into edinbrick1 failed. (YAN & OG)
