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Edinburgh/5 August 2008
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Revision as of 11:30, 20 August 2008
Week 8
Tuesday 5 August 08
- M72 (pSB1A2+rbs+dxs), M79 (BABEL2+crtE) and M82 (pSB1A2+rbs+appY) submitted for seqencing. (CF)
- Minipreps M97-104 of pSB1A2 cenA (2, 7-13) and M105-108 Minipreps M105-108 of pSB1A2+cex. (Yan)
- M97-108 were double-digested with EcoRI/PstI and run on gel 37. (Yan)
- Purification and self-ligation of P50 (3rd mutagenesis of glgc-mut1,2) (L30). (HX)
- M68-69 (glgc-mut1,2 in pSB1A2) and M92 (pSB1A2+cex) were digested with a) PstI and b) EcoRI/PstI, but gel leaked. Entire process needs repeating tomorrow! (CF, OG)
- Digested P36 (glgC-mut1,2) with Edinbrick1 using Spe1/EcoR1 - to be continued tomorrow. (OG)
- Plate 93 created from subculture of plate 56 (M67, pSB1A2+rbs+crtB)
