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Edinburgh/7 July 2008
From 2008.igem.org
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| - | * Several more white colonies have grown on plate 19, but quite small and close together. Tried to sub these to '''Plate 21'''. | + | </style> |
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| + | :::: '''[[Edinburgh/6_July_2008|< Previous Entry]]''' | ||
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| + | == Week 4 == | ||
| + | === Monday 7 July 08 === | ||
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| + | * Several more white colonies have grown on plate 19 (pSB1A2+''appY''), but quite small and close together. Tried to sub these to '''Plate 21'''. | ||
* Nimisha and the team did two maxipreps - '''X1''' (J33201), and '''X2''' (the ''dxs'' clone of miniprep M2). X2 digested with HindIII (single restriction digest). | * Nimisha and the team did two maxipreps - '''X1''' (J33201), and '''X2''' (the ''dxs'' clone of miniprep M2). X2 digested with HindIII (single restriction digest). | ||
| - | * Purified the mutagenic PCR reactions P6 and P7, and set up self ligations ('''L6 and L7'''). | + | * Purified the mutagenic PCR reactions P6 (BABEL2+''glgC''-mut1) and P7 (BABEL2+''glgC''-mut2), and set up self ligations ('''L6 and L7'''). |
| - | * Sequence results for ''glgC'' clones M10 and M11 show that both are in reverse orientation. The result for the ''dxs'' clones M2 looks OK at first glance, but can't see restriction sites since sequencing primers pSB1A2insf1 and pSB1A2insr1 were used rather than pSB1A2f2 and pSB1A2r2. | + | * Sequence results for ''glgC'' clones M10 and M11 show that both are in reverse orientation. The result for the ''dxs'' clones M2 looks OK at first glance, but can't see restriction sites since sequencing primers pSB1A2insf1 and pSB1A2insr1 were used rather than pSB1A2f2 and pSB1A2r2.<br /> |
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| + | :::: '''[[Edinburgh/8_July_2008|Next Entry >]]''' | ||
Revision as of 11:44, 21 August 2008
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Week 4
Monday 7 July 08
- Several more white colonies have grown on plate 19 (pSB1A2+appY), but quite small and close together. Tried to sub these to Plate 21.
- Nimisha and the team did two maxipreps - X1 (J33201), and X2 (the dxs clone of miniprep M2). X2 digested with HindIII (single restriction digest).
- Purified the mutagenic PCR reactions P6 (BABEL2+glgC-mut1) and P7 (BABEL2+glgC-mut2), and set up self ligations (L6 and L7).
- Sequence results for glgC clones M10 and M11 show that both are in reverse orientation. The result for the dxs clones M2 looks OK at first glance, but can't see restriction sites since sequencing primers pSB1A2insf1 and pSB1A2insr1 were used rather than pSB1A2f2 and pSB1A2r2.
