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Edinburgh/10 July 2008
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(New page: <html> <head> <title>Edinburgh iGEM 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07' var ta...) |
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| - | ==== Thursday 10 July 08 | + | <html> |
| + | <style type="text/css"> | ||
| + | table.calendar { margin: 0; padding: 2px; } | ||
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| + | table.month .heading td { padding:1px; background-color: black; color: white; text-align:center; font-size:140%; font-weight:bold; } | ||
| + | table.month .dow td { color:#000000; text-align:center; font-size:100%; } | ||
| + | table.month td.today { background-color:#cd0000; } | ||
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| + | padding: 0pt 0.5pt; | ||
| + | font-weight: bold; | ||
| + | font-size: 9pt; | ||
| + | text-align: right; | ||
| + | background-color: white; | ||
| + | } | ||
| + | #bodyContent table.month a { background:none; padding:0 } | ||
| + | .day-active { color:#cd0000 } | ||
| + | .day-empty { color:#000000 } | ||
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| + | </style> | ||
| + | </html> | ||
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| + | {|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:"center" width="250px" | ||
| + | </table> | ||
| + | |||
| + | {| align="left" | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}} | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}} | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | ||
| + | |} | ||
| + | |||
| + | |||
| + | :::: '''[[Edinburgh/9_July_2008|< Previous Entry]]''' | ||
| + | |||
| + | == Week 4 == | ||
| + | === Thursday 10 July 08 === | ||
* Subitted M15 (possible pSB1A2+''appY'') for sequencing with primers pSB1A2insf2 and pSB1A2insr2. | * Subitted M15 (possible pSB1A2+''appY'') for sequencing with primers pSB1A2insf2 and pSB1A2insr2. | ||
* Minipreps '''M19 to M24''' of possible ''glgC'' mutants with EcoRI site 2 removed. EcoRI digests were run on '''Gel 11'''. The insert bands are a bit fuzzy, but it looks as though '''M19, M21 and M22''' may have lost the site, and M20, M23 and M24 may still have it. | * Minipreps '''M19 to M24''' of possible ''glgC'' mutants with EcoRI site 2 removed. EcoRI digests were run on '''Gel 11'''. The insert bands are a bit fuzzy, but it looks as though '''M19, M21 and M22''' may have lost the site, and M20, M23 and M24 may still have it. | ||
* PCR '''P11''' using ligation L8 as template to generate a fusion product of rbs+''dxs''. Run on '''Gel 11''', last lane. No product visible. | * PCR '''P11''' using ligation L8 as template to generate a fusion product of rbs+''dxs''. Run on '''Gel 11''', last lane. No product visible. | ||
| - | * Digested P8 and P9 (''crtB'' and ''crtI''-mut PCR products) with EcoRI/SpeI together with Edinbrick1, purified and ligated (ligations '''L9 and L10''') at 16°C overnight. | + | * Digested P8 and P9 (''crtB'' and ''crtI''-mut PCR products) with EcoRI/SpeI together with Edinbrick1, purified and ligated (ligations '''L9 and L10''') at 16°C overnight.<br /> |
| + | <br /> | ||
| + | |||
| + | :::: '''[[Edinburgh/11_July_2008|Next Entry >]]''' | ||
Revision as of 11:59, 21 August 2008
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Week 4
Thursday 10 July 08
- Subitted M15 (possible pSB1A2+appY) for sequencing with primers pSB1A2insf2 and pSB1A2insr2.
- Minipreps M19 to M24 of possible glgC mutants with EcoRI site 2 removed. EcoRI digests were run on Gel 11. The insert bands are a bit fuzzy, but it looks as though M19, M21 and M22 may have lost the site, and M20, M23 and M24 may still have it.
- PCR P11 using ligation L8 as template to generate a fusion product of rbs+dxs. Run on Gel 11, last lane. No product visible.
- Digested P8 and P9 (crtB and crtI-mut PCR products) with EcoRI/SpeI together with Edinbrick1, purified and ligated (ligations L9 and L10) at 16°C overnight.
