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Edinburgh/Notebook/Minipreps
From 2008.igem.org
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'''M19 to M24:''' BABEL2+''glgC'' clones after mutation of EcoRI site 2. EcoRI digests on *Gel 11* suggest that *M19, M21 and M22* may have lost the EcoRI site. (10.07.08)<br /> | '''M19 to M24:''' BABEL2+''glgC'' clones after mutation of EcoRI site 2. EcoRI digests on *Gel 11* suggest that *M19, M21 and M22* may have lost the EcoRI site. (10.07.08)<br /> | ||
'''M25 to M30:''' possible rbs+''dxs'' transformants from plate 34. (13.07.08)<br /> | '''M25 to M30:''' possible rbs+''dxs'' transformants from plate 34. (13.07.08)<br /> | ||
| - | '''M31 to M36:''' ''crtB'' clones.<br /> | + | '''M31 to M36:''' pSB1A2+''crtB'' clones from plate 35. (14.07.08)<br /> |
| - | '''M37 to M42:''' ''crtI'' clones.<br /> | + | '''M37 to M42:''' pSB1A2+''crtI'' clones from plate 36. (14.07.08)<br /> |
| - | '''M43 to M47:''' possible ''glgC'' double mutants (both EcoRI sites gone)<br /> | + | '''M43 to M47:''' possible BABEL2+''glgC'' double mutants (both EcoRI sites gone). (19.07.08)<br /> |
| - | '''M48:''' a possible rbs+''dxs'' clone.<br /> | + | '''M48:''' a possible pSB1A2+rbs+''dxs'' clone. (19.07.08)<br /> |
'''M49 to M54:''' pSB1A2-rbs+''crtI''<br /> | '''M49 to M54:''' pSB1A2-rbs+''crtI''<br /> | ||
'''M55 to M60:''' PZntA promoter<br /> | '''M55 to M60:''' PZntA promoter<br /> | ||
Revision as of 16:33, 21 August 2008
M1 to M6: pSB1A2+dxs transformants (plate 11). M2, M3, M4 and M6 all looked correct on gel 2. (30.06.08) - M2 was sequenced to confirm identity and was maxiprepped as X2.
M7: BABEL1+glgC transformant (plate 12). Did not look right on gel. (30.06.08)
M8 to M12: BABEL2+glgC transformants (plate 13). M10 and M11 both looked good on a gel, but sequencing showed that both had the insert in the reverse orientation. (30.06.08)
M13 to M18: pSB1A2+appY transformants. Gel 10 showed that M15 to M18, and possibly M14, all seemed to have inserts the right size. (09.07.08)
M19 to M24: BABEL2+glgC clones after mutation of EcoRI site 2. EcoRI digests on *Gel 11* suggest that *M19, M21 and M22* may have lost the EcoRI site. (10.07.08)
M25 to M30: possible rbs+dxs transformants from plate 34. (13.07.08)
M31 to M36: pSB1A2+crtB clones from plate 35. (14.07.08)
M37 to M42: pSB1A2+crtI clones from plate 36. (14.07.08)
M43 to M47: possible BABEL2+glgC double mutants (both EcoRI sites gone). (19.07.08)
M48: a possible pSB1A2+rbs+dxs clone. (19.07.08)
M49 to M54: pSB1A2-rbs+crtI
M55 to M60: PZntA promoter
M61 to M66: BABEL2+rbs+crtE
M67: rbs+crtB
M68 to M71: pSB1A2+glgC-mut1,2 (31.07.08: AM, YAN)
M72 to M75: pSB1A2+rbs+dxs (31.07.08: AM, YAN)
M76 to M79: BABEL2+crtE (31.07.08: AM, YAN)
M80 to M83: pSB1A2+rbs+appY (04/08/08: YAN and OG)
M84 to M85: pSB1A2+rbs+crtB (04/08/08: YAN and OG)
M86 to M90: pSB1A2+cenA (04/08/08: YAN and OG)
M91 to M96: pSB1A2+cex(04/08/08: YAN and OG)
M97 to M104: pSB1A2+cenA (05.08.08: YAN)
M105 to M108: pSB1A2+cex (05.08.08: YAN)
